Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Phosphate Buffered Saline-Tween, PBST (20X, with 1% Tween-20, pH 7.4) - 4x1L
Phosphate Buffered Saline-Tween PBST (20X with 1% Tween-20 pH 7 4) - 4x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc MES Buffer (1 M, pH 5.0) - 500ml
MES Buffer (1 M pH 5 0) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.6) - 4L
Phosphate Buffered Saline PBS (10X pH 7 6) - 4L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffered Saline (5X, pH 7.4) - 500ml
HEPES Buffered Saline (5X pH 7 4) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Bis-Tris Transfer Buffer (20X) - 1L
Bis-Tris Transfer Buffer (20X) - 1L (For Research Use and Further Manufacturing Only)
Medchemexpress LLC Cetuximab PBS 5mg | 5mg
Cetuximab (PBS) is a human IgG1 monoclonal antibody that can inhibit EGFR The SPR method measured the Kd value of Cetuximab for EGFR to be 0 201 nM Cetuximab has a highly effective anti-tumor effect[1]
Boston Bioproducts Inc MES Buffer (0.5 M, pH 6.0) - 250ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Sigma Aldrich Fine Chemicals Biosciences Trizma base solution | 77-86-1 | MFCD00004679 | 100 mL
Trizma base solution | Mol Wt: 121.135 | 77-86-1 | MFCD00004679 | 100 mL
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4) - 1L
Tris-HCl Buffer (1 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
LICOR PBS Blocking Buffer and PVDF Kit (Single buffer kit)
The non-mammalian Intercept Blocker in these kits is in PBS (phosphate-buffered saline) containing 0.06% Kathon II. The Immobilon-FL PVDF membrane included in these kits has been tested to ensure a low-background for infrared Western blot success. This kit contains Intercept (PBS) Blocking Buffer (500 mL) and Millipore Immobilon-FL PVDF Membrane (1 roll; 0.45µm, 26.5 cm x 3.75 m)