Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Teknova 10X DPBS Concentrate without Calcium and Magnesium Salts. pH 7.4. 1Liter Bottle. Non Sterile. [H0] 2 pack.](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502387606.png-250.jpg)
Teknova 10X DPBS Concentrate without Calcium and Magnesium Salts. pH 7.4. 1Liter Bottle. Non Sterile. [H0] 2 pack.
1.54M Sodium Chloride,55mM Sodium Phosphate Dibasic,12mM Potassium Phosphate Monobasic
Alkali Scientific CellPro™ PBS Buffer, 1X pH 7.2, 100mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
![Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 500 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/default.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 500 Tablets per Bottle
PBS [Phosphate Buffered Saline] 100 mL Tablets are designed to make buffer preparation simple and efficient. Each tablet dissolves easily in water to create 100 mL of phosphate-buffered saline. With 500 tablets per bottle, these tablets are perfect for laboratories with high-volume buffer preparation needs. The ease of use and consistency of the PBS solution make these tablets ideal for various applications, including cell culture, microbiological analysis, and molecular biology experiments. The tablets provide a reliable way to ensure that each PBS solution is accurately prepared with the correct pH and osmolarity, minimizing the potential for human error in buffer formulation.
Apexbio Technology LLC Annexin V-Cy5/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Under normal conditions PS resides on the inner side of the cell membrane lipid bilayer However in early apoptosis PS translocates from the inner leaflet of the plasma membrane to the outer surface becoming exposed externally Annexin V exhibits high binding affinity for PS and when conjugated with fluorescent molecules (Cy5) it serves as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy The staining procedure requires only 10 20 minutes PI specifically binds to double-stranded DNA to produce red fluorescence it is normally impermeant to intact cell membranes However during late-stage apoptosis or in dead cells PI penetrates the membrane to stain nuclear DNA Combined Annexin V-Cy5 and PI staining allows distinction between cells at various stages of apoptosis and dead cells
![Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661850.jpg-250.jpg)
Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters
DPBS 1X (Dulbecco's Phosphate Buffered Saline) is a sterile saline solution used in biological research and clinical laboratories. The 500mL bottle is a convenient size for smaller laboratories or specific experiments that require moderate volumes of buffer solution. DPBS is widely used for washing cells, preparing tissues, and diluting reagents in cell culture, molecular biology, and microbiology applications. The balanced composition of salts and phosphate ensures that the solution maintains osmotic pressure and pH stability, making it essential for maintaining cell viability and supporting biological assays that require precise environmental conditions.
Biotium Fixation Permeabilization Other Buffers Buffers Accessories and Background Reducers Fixation Permeabilization and Other Buffers Chemical Reagents 50/PK
PBS (phosphate-buffered saline) buffer powder packet for preparing 2 L of 1X PBS buffer Box includes 50 packets
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbeccos Phosphate Bufer500ML
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Dulbeccos Modified Eagles500ML
Ethos Biosciences Dilurex 1X PBS Buffer, 1000ml, Ethos
Dilurex 1X PBS is a ready-to-use working concentration of pH 7.4 phosphate buffered saline. Skip the tedium of weighing powders and stocking raw materials that can expire. Our PBS is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences 1X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today.
![Alkali Scientific TES, Sodium Salt, [2 Tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid, sodium salt], 1 Kilogram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660867.jpg-250.jpg)
Alkali Scientific TES, Sodium Salt, [2 Tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid, sodium salt], 1 Kilogram
TES, Sodium Salt, is a high-purity buffering agent available in a 1-kilogram package. This tris(hydroxymethyl) methyl 2 amino 1 ethanesulfonic acid sodium salt is widely used in biochemical and molecular biology research for maintaining stable pH conditions in solutions. TES buffers are particularly effective in biological and clinical applications where precise pH control is essential. The high purity of this reagent makes it suitable for sensitive experiments, including enzyme assays, cell culture, and PCR reactions. TES buffers are used to stabilize proteins, DNA, and other biomolecules, ensuring consistent and reliable results in a variety of laboratory protocols.
eMolecules 602-25-5 | 1,4-DICHLOROANTHRAQUINONE | AstaTech | MFCD00192002 | 277.100 | C14H6Cl2O2 | 98.000 | Clc1ccc(Cl)c2C(=O)c3ccccc3C(=O)c12 | 0.25g | 475589680
1,4-DICHLOROANTHRAQUINONE | AstaTech | 602-25-5 | MFCD00192002 | 277.100 | C14H6Cl2O2 | 98.000 | Clc1ccc(Cl)c2C(=O)c3ccccc3C(=O)c12 | 0.25g | 475589680
Medchemexpress LLC Mops | 1132-61-2 | 99.95% | 25 G
MOPS is a buffering agent used in biology. It is known for its ability to maintain the pH of mammalian cell culture media and significantly improve the thermal stability of BSA.
- Buffering agent for biological applications.
- Maintains pH of mammalian cell culture media.
- Improves thermal stability of BSA.