Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.8) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Ethos Biosciences Dilurex 1M Tris-HCL Buffer, 1000ml, Ethos
Dilurex 1M Tris-HCL 10X Buffer is a classic laboratory buffer at pH 7.4. It does not include a saline ionic component and is free to be mixed with other components as needed in your laboratory. kip the tedium of weighing powders and stocking raw materials that can expire. Our buffer is convenient, ready to use, and will save you hours in the lab, letting you focus on breakthroughs rather than buffer prep. Thousands of researchers trust Ethos Biosciences buffers for their proven track record in top-tier publications and protocols - upgrade your productivity today.
Medchemexpress LLC Nvp-tae 226 | 761437-28-9 | MFCD12031516 | 99.8% | 468.94 g·mol⁻¹ | C23H25ClN6O3 | 5 MG
NVP-TAE 226 is an ATP-competitive small-molecule inhibitor of focal adhesion kinase (FAK) and insulin-like growth factor 1 receptor (IGF-1R), used in cellular and preclinical cancer research to probe signaling pathways and evaluate effects on proliferation, migration, and survival.
- Potent inhibition of FAK and Pyk2 with low-nanomolar IC50s.
- Also inhibits IGF-1R and insulin receptor, enabling study of connected signaling networks.
- Suitable for in vitro assays and preclinical efficacy models.
- Available as solid and solution formulations for flexible dosing.
- High purity appropriate for research applications.
- Stable under recommended storage conditions for prolonged shelf life.
Kd Medical Inc TAE (TRIS-ACETATE EDTA), 50X
TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. 50X concentrated ready to be diluted to a 1X working solution. One 500ml bottle.
Sigma Aldrich Fine Chemicals Biosciences mPAGER 8 Bis-Tris Precast
mPAGE 8% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Biotang Inc HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545
HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545.
Apexbio Technology LLC Annexin V-HF647/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes PI is a dye which specifically binds double-stranded DNA and emits red fluorescence under normal circumstances it cannot penetrate intact cell membranes However in late apoptotic and dead cells PI penetrates the cellular membrane staining the nuclei Dual staining with Annexin V-HF647 and PI enables clear differentiation between early apoptotic late apoptotic and dead cells
Seracare Life Sciences Inc 10X DULBECCO S PBS (D-PBS)
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. pH=7.4. Conductivity controlled.
Alkali Scientific CellPro™ DPBS Buffer, 1X, with Calcium and Magnesium, 500mL, 10/Case
Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. These uses include substance dilution and cell container rinsing.
Scytek Laboratories ScyTek Phosphate Buffered Saline (25x), 7.6 pH, 1000ml
ScyTek Phosphate Buffered Saline (25x) pH of 7.6 is an optimal formulation of pH stabilizers and salts designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal. Our Wash buffers do not contain hazardous preservatives such as Azide or Mercury that may interfere with antibody-antigen binding interactions. For your convenience wash buffer is offered in a wide variety of formulations to meet the needs of your specific ELISA application. This product can be customized to meet the specific needs of your assay. Inquire about custom vialing, labeling, kit assembly and drop shipping.
Apexbio Technology LLC Annexin V-Cy3/SYTOX Green Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane lipid bilayer but flips from the inner to the outer leaflet during the early stages of apoptosis becoming exposed to the extracellular environment Annexin V has a high affinity for PS By conjugating Annexin V with Cy3 the labeled Annexin V serves as a fluorescent probe enabling detection of apoptosis events via flow cytometry or fluorescence microscopy within only 10 20 minutes staining SYTOX Green is a membrane-impermeable cyanine dye that normally cannot penetrate intact cell membranes However during late-stage apoptosis or necrosis SYTOX Green can cross disrupted regions of the cell membrane reach the nucleus and bind DNA double helix molecules forming SYTOX Green-DNA complexes Binding increases fluorescence intensity over 500-fold generating bright green fluorescence Thus SYTOX Green stains o
Apexbio Technology LLC One-step TUNEL Cy5 Apoptosis Detection Kit 20rxns
One-step TUNEL Cy5 Apoptosis Detection Kit is designed for detecting cell nuclear DNA fragmentation in tissues or cells undergoing apoptosis During apoptosis intracellular DNA endonucleases are activated cleaving genomic DNA between nucleosomes and resulting in DNA fragmentation into segments approximately 180-200 bp or multiples thereof The fragmented genomic DNA contains exposed 3 -OH ends which are labeled with fluorescent-tagged dUTP in a TdT (Terminal Deoxynucleotidyl Transferase) catalyzed reaction allowing visualization and detection by fluorescence microscopy or flow cytometry (Ex/Em 649 nm/670 nm) This kit has a broad range of applications and can be utilized to detect apoptosis in frozen or paraffin-embedded tissue sections as well as in cultured adherent or suspension cells